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  • Featured Product
  • KD/KO Validated

ERCC3 Polyclonal antibody

ERCC3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10580-1-AP

Synonyms

EC:5.6.2.4, DNA repair protein complementing XP-B cells, DNA excision repair protein ERCC-3, DNA 3'-5' helicase/translocase XPB, BTF2 p89

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using ERCC3 Polyclonal antibody (10580-1-AP)

    WB analysis of HEK-293 using 10580-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using ERCC3 Polyclonal antibody (10580-1-AP)

    WB analysis of COLO 320 using 10580-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using ERCC3 Polyclonal antibody (10580-1-AP)

    WB analysis of HT-1080 using 10580-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using ERCC3 Polyclonal antibody (10580-1-AP)

    WB analysis of A431 using 10580-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ERCC3 Polyclonal antibody (10580-1-AP)

    WB analysis of HeLa using 10580-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ERCC3 Polyclonal antibody (10580-1-AP)

    WB analysis of HeLa using 10580-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using ERCC3 Polyclonal antibody (10580-1-AP)

    WB analysis of mouse colon using 10580-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ERCC3 Polyclonal antibody (10580-1-AP)

    WB analysis of HeLa using 10580-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ERCC3 Polyclonal antibody (10580-1-AP)

    WB analysis of HEK-293 using 10580-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A431 cells using ERCC3 Polyclonal antibody (10580-1-AP)

    IP experiment of A431 using 10580-1-AP

    IP result of anti-ERCC3 (IP:10580-1-AP, 4ug; Detection:10580-1-AP 1:500) with A431 cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human stomach tissue using ERCC3 Polyclonal antibody (10580-1-AP)

    IHC staining of human stomach using 10580-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10580-1-AP (ERCC3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, A431 cells, COLO 320 cells, HT-1080 cells, mouse colon tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10580-1-AP targets ERCC3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERCC3 fusion protein Ag0876
Full Name excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing)
Calculated Molecular Weight 89 kDa
Observed Molecular Weight 89 kDa
GenBank Accession NumberBC008820
Gene Symbol ERCC3
Gene ID (NCBI) 2071
RRIDAB_2262174
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Excision repair cross-complementing 3/xeroderma pigmentosum B (ERCC3/XPB) DNA helicase, is an essential ATP-dependent DNA helicase that functions as an integral component of the transcription factor IIH complex. ERCC3 is involved in initiation of basal transcription and global genome repair as well as in transcription-coupled repair (TCR) [PMID:19942596]. At the cellular level, a defect in ERCC3 results in high UV sensitivity, reduced post-UV RNA synthesis recovery and decreased DNA repair due to defective NER [PMID:16947863].

Protocols

Product Specific Protocols
WB protocol for ERCC3 antibody 10580-1-APDownload protocol
IHC protocol for ERCC3 antibody 10580-1-APDownload protocol
IP protocol for ERCC3 antibody 10580-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Theranostics

Triptolide Inhibits the AR Signaling Pathway to Suppress the Proliferation of Enzalutamide Resistant Prostate Cancer Cells.

Authors - Yangyang Han
  • KD Validated
View Article
humanWB

Biochem Biophys Res Commun

MiR-192 inhibits nucleotide excision repair by targeting ERCC3 and ERCC4 in HepG2.2.15 cells.

Authors - Xie Qiong-Hui QH
View Article
humanWB

Science

Structural visualization of transcription initiation in action

Authors - Xizi Chen
View Article

Mol Cell

Three-step mechanism of promoter escape by RNA polymerase II

Authors - Yumeng Zhan
View Article