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ERC2 Polyclonal antibody

ERC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21396-1-AP

Synonyms

KIAA0378, ERC protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using ERC2 Polyclonal antibody (21396-1-AP)

    WB analysis of human brain using 21396-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21396-1-AP (ERC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using ERC2 Polyclonal antibody (21396-1-AP)

    WB analysis of rat brain using 21396-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 21396-1-AP (ERC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human bowen disease using ERC2 Polyclonal antibody (21396-1-AP)

    IHC staining of human bowen disease using 21396-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 21396-1-AP (ERC2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease using ERC2 Polyclonal antibody (21396-1-AP)

    IHC staining of human bowen disease using 21396-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 21396-1-AP (ERC2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ERC2 Polyclonal antibody (21396-1-AP)

    IF Staining of HeLa using 21396-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ERC2 antibody (21396-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inhuman brain tissue, rat brain tissue
Positive IHC detected inhuman bowen disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21396-1-AP targets ERC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERC2 fusion protein Ag16080
Full Name ELKS/RAB6-interacting/CAST family member 2
Calculated Molecular Weight 957 aa, 111 kDa
Observed Molecular Weight 115-120 kDa
GenBank Accession NumberBC111550
Gene Symbol ERC2
Gene ID (NCBI) 26059
RRIDAB_10859782
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ERC2 antibody 21396-1-APDownload protocol
IHC protocol for ERC2 antibody 21396-1-APDownload protocol
IF protocol for ERC2 antibody 21396-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols