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  • Featured Product
  • KD/KO Validated

ERBB2IP Polyclonal antibody

ERBB2IP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22438-1-AP

Synonyms

LAP2, KIAA1225, ERBIN, Erbb2-interacting protein, Densin-180-like protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ERBB2IP Polyclonal antibody (22438-1-AP)

    WB analysis using 22438-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22438-1-AP (ERBB2IP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ERBB2IP Polyclonal antibody (22438-1-AP)

    WB analysis of MCF-7 using 22438-1-AP

    WB result of ERBB2IP antibody (22438-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERBB2IP transfected MCF-7 cells.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ERBB2IP Polyclonal antibody (22438-1-AP)

    IHC staining of human kidney using 22438-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22438-1-AP (ERBB2IP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ERBB2IP Polyclonal antibody (22438-1-AP)

    IHC staining of human kidney using 22438-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22438-1-AP (ERBB2IP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of H9C2 cells using ERBB2IP Polyclonal antibody (22438-1-AP)

    IF Staining of H9C2 using 22438-1-AP

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using ERBB2IP antibody (22438-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inH9C2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22438-1-AP targets ERBB2IP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERBB2IP fusion protein Ag18206
Full Name erbb2 interacting protein
Calculated Molecular Weight 1412 aa, 158 kDa
Observed Molecular Weight 150-180 kDa
GenBank Accession NumberBC115012
Gene Symbol ERBB2IP
Gene ID (NCBI) 55914
RRIDAB_2879105
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERBB2IP is a cytoplasmic protein that contains leucine-rich repeats (LRR) and PSD95/Dlg1/zo-1 (PDZ) domain (thus named a LAP protein). ERBB2IP interacts, via its single PDZ domain, specifically with ErbB2, but not with ErbB3, ErbB4, or EGFR. It is colocalized with ErbB2 at the basolateral membranes of epithelial cells. In addition, ERBB2IP also interacts with PSD‐95, Lano and with three p120‐related catenins, eBPAG1 and the β4 subunit of the α6β4 integrin, two major components of hemidesmosomes. The molecular weight of ERBB2IP was predicted 150~180 kDa by Western Blot.(PMID: 25288731,PMID: 24252174,PMID: 19289088)

Protocols

Product Specific Protocols
WB protocol for ERBB2IP antibody 22438-1-APDownload protocol
IHC protocol for ERBB2IP antibody 22438-1-APDownload protocol
IF protocol for ERBB2IP antibody 22438-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

J Exp Med

ERBIN deficiency links STAT3 and TGF-β pathway defects with atopy in humans.

Authors - J J Lyons
View Article
humanWB,IHC

Oncol Lett

Reduced IκBα promotes hepatocellular carcinoma cell proliferation and migration via regulation of NF-κB/Erbin axis.

Authors - Ye Tan
View Article
humanWB

Mol Cancer Res

Desmoglein 1 regulates invadopodia by suppressing EGFR/Erk signaling in an Erbin-dependent manner.

Authors - Alejandra Valenzuela-Iglesias
View Article