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EPHX3 Polyclonal antibody

EPHX3 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25608-1-AP

Synonyms

EH3, EC:3.3.2.10, Abhydrolase domain-containing protein 9, ABHD9

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EPHX3 Polyclonal antibody (25608-1-AP)

    IHC staining of human breast cancer using 25608-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25608-1-AP (EPHX3 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using EPHX3 Polyclonal antibody (25608-1-AP)

    IF Staining of A549 using 25608-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using EPHX3 antibody (25608-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25608-1-AP targets EPHX3 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EPHX3 fusion protein Ag22277
Full Name epoxide hydrolase 3
Calculated Molecular Weight 360 aa, 41 kDa
GenBank Accession NumberBC132960
Gene Symbol EPHX3
Gene ID (NCBI) 79852
RRIDAB_2880158
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for EPHX3 antibody 25608-1-APDownload protocol
IF protocol for EPHX3 antibody 25608-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols