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  • Western Blot (WB) analysis of various lysates using ELOF1 Polyclonal antibody (21070-1-AP)

    WB analysis using 21070-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21070-1-AP (ELOF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ELOF1 Polyclonal antibody (21070-1-AP)

    WB analysis using 21070-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21070-1-AP (ELOF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

ELOF1 Polyclonal antibody

ELOF1 Polyclonal Antibody for WB, ELISA
Cat No. 21070-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ELF1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inmouse testis tissue, HEK-293 cells, rat testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21070-1-AP targets ELOF1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ELOF1 fusion protein Ag15213 Predict reactive species
Full Name elongation factor 1 homolog (S. cerevisiae)
Calculated Molecular Weight9.5 kDa
Observed Molecular Weight 9 kDa
GenBank Accession NumberBC007516
Gene Symbol ELOF1
Gene ID (NCBI) 84337
RRIDAB_10693632
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP60002
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ELOF1 (elongation factor 1 homologue) is a small (83 aa), evolutionarily conserved, RNAPII-associated transcription elongation factor that is also a core component of the transcription-coupled DNA repair machinery with an additional role in preventing DNA damage during DNA replication (PMID: 34108663). Loss of ELOF1 strongly compromises AID-mediated deamination, SHM, and CSR, and that this is accompanied by a decrease in parameters of RNAPII pausing/stalling and in RNAPII RNA synthesis without a decrease in levels of S2P-RNAPII (PMID: 39386505).

Protocols

Product Specific Protocols
WB protocol for ELOF1 antibody 21070-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols