mouse brain tissue were subjected to SDS PAGE followed by western blot with 24992-1-AP (HuD-specific antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 24992-1-AP (HuD-specific antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 24992-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 24992-1-AP (HuD-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
rat brain tissue were subjected to SDS PAGE followed by western blot with 24992-1-AP (HuD-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 24992-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 24992-1-AP (HuD-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 24992-1-AP (HuD-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 24992-1-AP
IP result of anti-HuD-specific (IP:24992-1-AP, 4ug; Detection:24992-1-AP 1:300) with mouse brain tissue lysate 3600ug.
IP result of anti-HuD-specific (IP:24992-1-AP, 4ug; Detection:24992-1-AP 1:300) with mouse brain tissue lysate 3600ug.
IHC staining of human brain using 24992-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24992-1-AP (HuD-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24992-1-AP (HuD-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 24992-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24992-1-AP (HuD-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24992-1-AP (HuD-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 24992-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using HuD-specific antibody (24992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using HuD-specific antibody (24992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of PC-12 using 24992-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using HuD-specific antibody (24992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using HuD-specific antibody (24992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
mouse brain tissue
Positive IF/ICC detected in
PC-12 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:4000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)-P
IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
ELAVL4, also named as HuD, is a 380 amino acid protein, which belongs to the RRM elav family. ELAVL4 may play a role in neuron-specific RNA processing and protects CDKN1A mRNA from decay by binding to its 3'-UTR. PC12 cells expressing T7±HuD were treated with thio-specific (BMH) or amine-specific (DSS) chemical crosslinking reagents, and the resultant crosslinked complexes in the cell extracts were analyzed by immunoblotting with anti-T7 antibody. In addition to the monomeric HuD of 41 kDa, three specific crosslinked complexes could be detected using either of the crosslinkers. Two of the complexes had molecular masses of ~80 and 120 kDa, and were likely to be the dimer and trimer of HuD, respectively. (PMID: 12384599 )
mouse brain tissue were subjected to SDS PAGE followed by western blot with 24992-1-AP (HuD-specific antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 24992-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 24992-1-AP (HuD-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 24992-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 24992-1-AP (HuD-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human brain using 24992-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24992-1-AP (HuD-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 24992-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24992-1-AP (HuD-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of mouse brain using 24992-1-AP
IP result of anti-HuD-specific (IP:24992-1-AP, 4ug; Detection:24992-1-AP 1:300) with mouse brain tissue lysate 3600ug.
IF-P Figures
IF Staining of mouse brain using 24992-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using HuD-specific antibody (24992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF/ICC Figures
IF Staining of PC-12 using 24992-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using HuD-specific antibody (24992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
HuD-specific Polyclonal antibody
Catalog Number
24992-1-AP
Citations
3
Dilutions
WB : 1:1000-1:4000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF-P : 1:50-1:500 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IF-P, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
