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EIF2B2 Polyclonal antibody

Name: EIF2B2 Polyclonal antibody
Brand: Proteintech
SKU: 11034-1-AP

EIF2B2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11034-1-AP

Synonyms

EIF 2Bbeta, EIF2B, EIF2B2, EIF2BB, S20I15, S20III15

Validation Data Gallery

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AllWBIPIHC

WB analysis using 11034-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 11034-1-AP (EIF2B2 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.
WB analysis using 11034-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11034-1-AP (EIF2B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11034-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11034-1-AP (EIF2B2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 11034-1-AP
IP result of anti-EIF2B2 (IP:11034-1-AP, 4ug; Detection:11034-1-AP 1:500) with HEK-293 cells lysate 2000 ug.
IHC staining of rat brain using 11034-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11034-1-AP (EIF2B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 11034-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11034-1-AP (EIF2B2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 11034-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11034-1-AP (EIF2B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 11034-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11034-1-AP (EIF2B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 11034-1-AP

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Tested Applications

Positive WB detected in	A431 cells, mouse liver tissue, HEK-293 cells, K-562 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	rat brain tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:14000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 7 publications below
IF	See 3 publications below

Product Information

11034-1-AP targets EIF2B2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EIF2B2 fusion protein Ag1502
Full Name	eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa
Calculated Molecular Weight	39 kDa
Observed Molecular Weight	39 kDa
GenBank Accession Number	BC011750
Gene Symbol	EIF2B2
Gene ID (NCBI)	8892
RRID	AB_2096016
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EIF2B2 antibody 11034-1-AP	Download protocol
IHC protocol for EIF2B2 antibody 11034-1-AP	Download protocol
IP protocol for EIF2B2 antibody 11034-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Elife Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response. Authors - Carmela Sidrauski View Article
	WB	Elife The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes. Authors - Yao Liang Wong View Article
human	WB	Elife eIF2B conformation and assembly state regulate the integrated stress response. Authors - Michael Schoof View Article
human	WB	Elife A point mutation in the nucleotide exchange factor eIF2B constitutively activates the integrated stress response by allosteric modulation. Authors - Morgane Boone View Article
human	WB	RNA EIF2B2 mutations in Vanishing White Matter Disease hyper- suppress translation and delay recovery during the integrated stress response. Authors - Stephanie L Moon View Article
human	WB	Biochem J Stoichiometry of the eIF2B complex is maintained by mutual stabilization of subunits. Authors - Noel C Wortham KD Validated View Article

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EIF2B2 Polyclonal antibody

EIF2B2 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 11034-1-AP

WB analysis using 11034-1-AP

WB analysis of mouse liver using 11034-1-AP

IP experiment of HEK-293 using 11034-1-AP

IHC staining of rat brain using 11034-1-AP

IHC staining of rat brain using 11034-1-AP

IHC staining of mouse brain using 11034-1-AP

IHC staining of mouse brain using 11034-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response.

The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes.

eIF2B conformation and assembly state regulate the integrated stress response.

A point mutation in the nucleotide exchange factor eIF2B constitutively activates the integrated stress response by allosteric modulation.

EIF2B2 mutations in Vanishing White Matter Disease hyper- suppress translation and delay recovery during the integrated stress response.

Stoichiometry of the eIF2B complex is maintained by mutual stabilization of subunits.