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EHMT2/G9a Polyclonal antibody

EHMT2/G9a Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 11595-1-AP

Synonyms

BAT8, C6orf30, EHMT2, EHMT2/G9a, G9A, H3 K9 HMTase 3, HLA B associated transcript 8, KMT1C, Lysine N methyltransferase 1C, NG36, NG36/G9a, Protein G9a

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using EHMT2/G9a Polyclonal antibody (11595-1-AP)

    WB analysis of Jurkat using 11595-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11595-1-AP (EHMT2/G9a antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using EHMT2/G9a Polyclonal antibody (11595-1-AP)

    WB analysis of HEK-293 using 11595-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11595-1-AP (EHMT2/G9a antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using EHMT2/G9a Polyclonal antibody (11595-1-AP)

    WB analysis of Jurkat using 11595-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11595-1-AP (EHMT2/G9a antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using EHMT2/G9a Polyclonal antibody (11595-1-AP)

    WB analysis of HeLa using 11595-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11595-1-AP (EHMT2/G9a antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11595-1-AP targets EHMT2/G9a in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EHMT2/G9a fusion protein Ag2171
Full Name euchromatic histone-lysine N-methyltransferase 2
Calculated Molecular Weight 1210 aa, 132 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC018718
Gene Symbol EHMT2
Gene ID (NCBI) 10919
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EHMT2/G9a antibody 11595-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Exp Gerontol

Age-related changes in histone modification in rat gastrocnemius muscle.

Authors - Toshinori Yoshihara
View Article
humanWB

Mol Cell Biochem

Histone methyltransferase G9a and H3K9 dimethylation inhibit the self-renewal of glioma cancer stem cells.

Authors - Hong Tao
View Article
humanWB

Cell Rep

Branched-Chain Amino Acid Metabolic Reprogramming Orchestrates Drug Resistance to EGFR Tyrosine Kinase Inhibitors.

Authors - Yuetong Wang
  • KD Validated
View Article