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DPPA2 Polyclonal antibody

DPPA2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12689-1-AP

Synonyms

ECSA, Pluripotent embryonic stem cell-related gene 1 protein, PESCRG1, Developmental pluripotency-associated protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using DPPA2 Polyclonal antibody (12689-1-AP)

    WB analysis of MCF-7 using 12689-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12689-1-AP (DPPA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using DPPA2 Polyclonal antibody (12689-1-AP)

    WB analysis of HepG2 using 12689-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12689-1-AP (DPPA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using DPPA2 Polyclonal antibody (12689-1-AP)

    WB analysis of HepG2 using 12689-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12689-1-AP (DPPA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using DPPA2 Polyclonal antibody (12689-1-AP)

    WB analysis of MCF-7 using 12689-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 12689-1-AP (DPPA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DPPA2 Polyclonal antibody (12689-1-AP)

    IF Staining of A431 using 12689-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using 12689-1-AP (DPPA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMCF7 cells, HepG2 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

12689-1-AP targets DPPA2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DPPA2 fusion protein Ag3601
Full Name developmental pluripotency associated 2
Calculated Molecular Weight 298 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC018070
Gene Symbol DPPA2
Gene ID (NCBI) 151871
RRIDAB_2277458
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DPPA2 antibody 12689-1-APDownload protocol
IF protocol for DPPA2 antibody 12689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Mol Hum Reprod

Differential expression of the embryo/cancer gene ECSA(DPPA2), the cancer/testis gene BORIS and the pluripotency structural gene OCT4, in human preimplantation development.

Authors - Monk Marilyn M
View Article