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DUT Polyclonal antibody

DUT Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13740-1-AP

Synonyms

EC:3.6.1.23, dUTPase, deoxyuridine triphosphatase, Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DUT Polyclonal antibody (13740-1-AP)

    WB analysis using 13740-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13740-1-AP (DUT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using DUT Polyclonal antibody (13740-1-AP)

    WB analysis of human liver using 13740-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13740-1-AP (DUT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using DUT Polyclonal antibody (13740-1-AP)

    WB analysis of human kidney using 13740-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 13740-1-AP (DUT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using DUT Polyclonal antibody (13740-1-AP)

    WB analysis of HEK-293 using 13740-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13740-1-AP (DUT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using DUT Polyclonal antibody (13740-1-AP)

    IP experiment of HEK-293 using 13740-1-AP

    IP result of anti-DUT (IP:13740-1-AP, 4ug; Detection:13740-1-AP 1:800) with HEK-293 cells lysate 1440 ug.

  • Immunohistochemistry (IHC) staining of human heart tissue using DUT Polyclonal antibody (13740-1-AP)

    IHC staining of human heart using 13740-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13740-1-AP (DUT Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using DUT Polyclonal antibody (13740-1-AP)

    IHC staining of human heart using 13740-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13740-1-AP (DUT Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DUT Polyclonal antibody (13740-1-AP)

    IF Staining of HeLa using 13740-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DUT antibody (13740-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, human liver tissue, human kidney tissue, HEK-293 cells, Ramos cells, A549 cells, HepG2 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13740-1-AP targets DUT in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DUT fusion protein Ag4677
Full Name deoxyuridine triphosphatase
Calculated Molecular Weight 252 aa, 27 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC033645
Gene Symbol DUT
Gene ID (NCBI) 1854
RRIDAB_2093170
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DUT antibody 13740-1-APDownload protocol
IHC protocol for DUT antibody 13740-1-APDownload protocol
IF protocol for DUT antibody 13740-1-APDownload protocol
IP protocol for DUT antibody 13740-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,CoIP

Cell Metab

Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.

Authors - Yi-Ping Wang
View Article
humanWB

Cell Chem Biol

CETSA interaction proteomics define specific RNA-modification pathways as key components of fluorouracil-based cancer drug cytotoxicity.

Authors - Ying Yu Liang
View Article
humanWB

Bioconjug Chem

ATP-Linked Chimeric Nucleotide as a Specific Luminescence Reporter of Deoxyuridine Triphosphatase.

Authors - Debin Ji
View Article