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  • Featured Product
  • KD/KO Validated

DTX4 Polyclonal antibody

DTX4 Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 25222-1-AP

Synonyms

Protein deltex 4, KIAA0937, EC:2.3.2.27, E3 ubiquitin-protein ligase DTX4, Deltex4

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using DTX4 Polyclonal antibody (25222-1-AP)

    WB analysis of mouse kidney using 25222-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25222-1-AP (DTX4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using DTX4 Polyclonal antibody (25222-1-AP)

    WB analysis of mouse kidney using 25222-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25222-1-AP (DTX4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse embryo tissue using DTX4 Polyclonal antibody (25222-1-AP)

    IF Staining of mouse embryo using 25222-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 25222-1-AP (DTX4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using DTX4 Polyclonal antibody (25222-1-AP)

    IF Staining of HEK-293 using 25222-1-AP

    Immunofluorescent analysis of HEK-293 cells using 25222-1-AP (DTX4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse kidney tissue
Positive IF-P detected inmouse embryo tissue
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25222-1-AP targets DTX4 in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DTX4 fusion protein Ag18415
Full Name deltex homolog 4 (Drosophila)
Calculated Molecular Weight 619 aa, 67 kDa
Observed Molecular Weight 67-70 kDa
GenBank Accession NumberBC119011
Gene Symbol DTX4
Gene ID (NCBI) 23220
RRIDAB_2879970
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DTX4, also named as KIAA0937, RNF155, is a 619 amino acid protein, which belongs to the Deltex family. DTX4 as a regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. DTX4 functions as a ubiquitin ligase protein in vivo, mediating 'Lys48'-linked polyubiquitination and promoting degradation of TBK1, targeting to TBK1 requires interaction with NLRP4.

Protocols

Product Specific Protocols
WB protocol for DTX4 antibody 25222-1-APDownload protocol
IF protocol for DTX4 antibody 25222-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Sci

Ubiquitination of NOTCH2 by DTX3 suppresses the proliferation and migration of human esophageal carcinoma.

Authors - Xin-Yu Ding
View Article
ratWB

Stem Cells Dev

Combined Methylome and Transcriptome Analysis During Rat Hepatic Stellate Cell Activation.

Authors - Eva Christine Schumacher
View Article
mouseWB,IF

Biochem Biophys Res Commun

E3 ubiquitin ligase DTX4 is required for adipogenic differentiation in 3T3-L1 preadipocytes cell line.

Authors - Zonggui Wang
  • KD Validated
View Article
mouseWB

Cell Death Dis

Gasdermin D silencing alleviates airway inflammation and remodeling in an ovalbumin-induced asthmatic mouse model

Authors - Jinxiang Wu
View Article