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  • Featured Product
  • KD/KO Validated

DSTYK Polyclonal antibody

DSTYK Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20102-1-AP

Synonyms

RIPK5, Receptor-interacting serine/threonine-protein kinase 5, KIAA0472, HDCMD38P, EC:2.7.12.1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DSTYK Polyclonal antibody (20102-1-AP)

    WB analysis using 20102-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20102-1-AP (DSTYK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DSTYK Polyclonal antibody (20102-1-AP)

    IF Staining of HepG2 using 20102-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DSTYK antibody (20102-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, rat liver tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20102-1-AP targets DSTYK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DSTYK fusion protein Ag13678
Full Name dual serine/threonine and tyrosine protein kinase
Calculated Molecular Weight 66 kDa, 105 kDa
Observed Molecular Weight66 kDa, 100~105 kDa
GenBank Accession NumberBC060870
Gene Symbol DSTYK
Gene ID (NCBI) 25778
RRIDAB_10694146
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DSTYK (Dual serine-threonine and tyrosine protein kinase gene), also known as CAKUT1, DustyPK, SPG23 has been classically considered as RIPK5, encodes a protein product consisting of 850-931 amino acids, which has a striking membrane-associated distribution in mesenchymal-derived cells of all major organs including brain, heart, kidney, lung, colon, and muscle (PMID: 34853310). DSTYK is strongly expressed in the cytoplasm of most malignant cells and that the high expression of DSTYK in human colon cancer specimens is associated with tumor progression and/or poor prognosis.

Protocols

Product Specific Protocols
WB protocol for DSTYK antibody 20102-1-APDownload protocol
IF protocol for DSTYK antibody 20102-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
monkeyIF

Nat Commun

Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway.

Authors - Xianding Sun
View Article
human,mouseWB,IHC

Cell Death Dis

Loss of DSTYK activates Wnt/β-catenin signaling and glycolysis in lung adenocarcinoma

Authors - Chenxi Zhong
  • KD Validated
View Article