DSN1 Polyclonal antibody
DSN1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 17742-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
17742-1-AP targets DSN1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DSN1 fusion protein Ag11988 |
| Full Name | DSN1, MIND kinetochore complex component, homolog (S. cerevisiae) |
| Calculated Molecular Weight | 340 aa, 38 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC026011 |
| Gene Symbol | DSN1 |
| Gene ID (NCBI) | 79980 |
| RRID | AB_2878431 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DSN1, also named as C20orf172 and MIS13, is required to shape an interface between the Mis12 complex and Blinkin, the counterpart of Spc105. DSN1 is part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. DSN1 has four isoforms with MW 27-33 kDa and 38-40 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DSN1 antibody 17742-1-AP | Download protocol |
| IHC protocol for DSN1 antibody 17742-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2. | ||
J Exp Clin Cancer Res Long noncoding RNA Linc00337 functions as an E2F1 co-activator and promotes cell proliferation in pancreatic ductal adenocarcinoma. |
