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DSE Monoclonal antibody

DSE Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2C9F7

Cat no : 60041-1-Ig

Synonyms

dermatan sulfate epimerase, DS epimerase, DSE, DSEPI, SART 2, SART2

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using DSE Monoclonal antibody (60041-1-Ig)

    WB analysis of COLO 320 using 60041-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60041-1-Ig (DSE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DSE Monoclonal antibody (60041-1-Ig)

    IHC staining of human liver cancer using 60041-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60041-1-Ig (DSE Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DSE Monoclonal antibody (60041-1-Ig)

    IHC staining of human liver cancer using 60041-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60041-1-Ig (DSE Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DSE Monoclonal antibody (60041-1-Ig)

    IF Staining of HeLa using 60041-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DSE antibody (60041-1-Ig, Clone: 2C9F7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inCOLO 320 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

60041-1-Ig targets DSE in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen DSE fusion protein Ag0695
Full Name dermatan sulfate epimerase
Calculated Molecular Weight 958 aa, 110 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC039245
Gene Symbol DSE
Gene ID (NCBI) 29940
RRIDAB_2093286
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DSE, also named as SART2 and DSEPI, is an enzyme that converts D-glucuronic acid to L-iduronic acid residues in dermatan sulphate biosynthesis. It is also identified to be a tumour-associated antigen. DSE is recognized by cytotoxic T cells (CTLs) and its enhanced expression in many cancers has been reported. DSE is a potential candidate for a tumour antigen with immunogenicity.

Protocols

Product Specific Protocols
WB protocol for DSE antibody 60041-1-IgDownload protocol
IHC protocol for DSE antibody 60041-1-IgDownload protocol
IF protocol for DSE antibody 60041-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Inflamm Regen

IFN-γ-STAT1-mediated CD8+ T-cell-neural stem cell cross talk controls astrogliogenesis after spinal cord injury

Authors - Jingyu Wang
View Article