• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

DRG1 Polyclonal antibody

DRG1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13190-1-AP

Synonyms

TRAFAC GTPase DRG1, Neural precursor cell expressed developmentally down-regulated protein 3, EC:3.6.5.-, DRG-1, DRG 1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DRG1 Polyclonal antibody (13190-1-AP)

    WB analysis using 13190-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13190-1-AP (DRG1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using DRG1 Polyclonal antibody (13190-1-AP)

    WB analysis of K-562 using 13190-1-AP

    WB result of DRG1 antibody (13190-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DRG1 transfected K-562 cells.

  • Western Blot (WB) analysis of PC-12 cells using DRG1 Polyclonal antibody (13190-1-AP)

    WB analysis of PC-12 using 13190-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 13190-1-AP (DRG1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using DRG1 Polyclonal antibody (13190-1-AP)

    IP experiment of K-562 using 13190-1-AP

    IP result of anti-DRG1 (IP:13190-1-AP, 4ug; Detection:13190-1-AP 1:600) with K-562 cells lysate 4400 ug.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using DRG1 Polyclonal antibody (13190-1-AP)

    IHC staining of human thyroid cancer using 13190-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 13190-1-AP (DRG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using DRG1 Polyclonal antibody (13190-1-AP)

    IHC staining of human thyroid cancer using 13190-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 13190-1-AP (DRG1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using DRG1 Polyclonal antibody (13190-1-AP)

    IHC staining of mouse kidney using 13190-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13190-1-AP (DRG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using DRG1 Polyclonal antibody (13190-1-AP)

    IHC staining of mouse kidney using 13190-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13190-1-AP (DRG1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using DRG1 Polyclonal antibody (13190-1-AP)

    IHC staining of rat kidney using 13190-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13190-1-AP (DRG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using DRG1 Polyclonal antibody (13190-1-AP)

    IHC staining of rat kidney using 13190-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13190-1-AP (DRG1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using DRG1 Polyclonal antibody (13190-1-AP)

    IHC staining of rat skeletal muscle using 13190-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 13190-1-AP (DRG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using DRG1 Polyclonal antibody (13190-1-AP)

    IHC staining of rat skeletal muscle using 13190-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 13190-1-AP (DRG1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DRG1 Polyclonal antibody (13190-1-AP)

    IHC staining of human colon cancer using 13190-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13190-1-AP (DRG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DRG1 Polyclonal antibody (13190-1-AP)

    IHC staining of human colon cancer using 13190-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13190-1-AP (DRG1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using DRG1 Polyclonal antibody (13190-1-AP)

    IHC staining of human placenta using 13190-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13190-1-AP (DRG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using DRG1 Polyclonal antibody (13190-1-AP)

    IHC staining of human placenta using 13190-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13190-1-AP (DRG1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DRG1 Polyclonal antibody (13190-1-AP)

    IF Staining of HepG2 using 13190-1-AP

    Immunofluorescent analysis of HepG2 cells using 13190-1-AP (DRG1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, PC-12 cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman thyroid cancer tissue, mouse kidney tissue, rat kidney tissue, rat skeletal muscle tissue, human colon cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13190-1-AP targets DRG1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DRG1 fusion protein Ag3866
Full Name developmentally regulated GTP binding protein 1
Calculated Molecular Weight 367 aa, 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC020803
Gene Symbol DRG1
Gene ID (NCBI) 4733
RRIDAB_2095275
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DRG1 antibody 13190-1-APDownload protocol
IHC protocol for DRG1 antibody 13190-1-APDownload protocol
IF protocol for DRG1 antibody 13190-1-APDownload protocol
IP protocol for DRG1 antibody 13190-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Chem Biol

The Jumonji-C oxygenase JMJD7 catalyzes (3S)-lysyl hydroxylation of TRAFAC GTPases.

Authors - Suzana Markolovic
View Article