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CRMP5 Polyclonal antibody

CRMP5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10525-1-AP

Synonyms

DPYSL5, DPYSL 5, DPYS L5, Dihydropyrimidinase-related protein 5, CRMP-5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CRMP5 Polyclonal antibody (10525-1-AP)

    WB analysis using 10525-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10525-1-AP (CRMP5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using CRMP5 Polyclonal antibody (10525-1-AP)

    IP experiment of mouse brain using 10525-1-AP

    IP result of anti-CRMP5 (IP:10525-1-AP, 4ug; Detection:10525-1-AP 1:2000) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CRMP5 Polyclonal antibody (10525-1-AP)

    IHC staining of mouse brain using 10525-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10525-1-AP (CRMP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CRMP5 Polyclonal antibody (10525-1-AP)

    IF Staining of SH-SY5Y using 10525-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CRMP5 antibody (10525-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10525-1-AP targets CRMP5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRMP5 fusion protein Ag0783
Full Name dihydropyrimidinase-like 5
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC002874
Gene Symbol CRMP5
Gene ID (NCBI) 56896
RRIDAB_2094644
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DPYSL5(Dihydropyrimidinase-related protein 5) is also named as CRMP5, ULIP6 and belongs to the hydantoinase/dihydropyrimidinase subfamily. It may have a function in neuronal differentiation and/or axon growth. SCLCs universally express CRMP5 and in thymoma, none of the CRMPs are detected by immunohistochemistry in tumorous epithelial cells with specific antibodies including CRMP5.The normal CRMP5 is probably not expressed by tumorous epithelial cells.(PMID:16519949).

Protocols

Product Specific Protocols
WB protocol for CRMP5 antibody 10525-1-APDownload protocol
IHC protocol for CRMP5 antibody 10525-1-APDownload protocol
IF protocol for CRMP5 antibody 10525-1-APDownload protocol
IP protocol for CRMP5 antibody 10525-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Proteomics

Network of brain protein level changes in glutaminase deficient fetal mice.

Authors - Bae Narkhyun N
View Article
humanWB,IHC

J Int Med Res

Ubiquitin C-terminal hydrolase L1 promotes lymph node metastasis in small cell neuroendocrine carcinomas of the cervix.

Authors - Yunqiang Zhang
View Article