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- KD/KO Validated
DPYS Polyclonal antibody
DPYS Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 13237-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IHC detected in | human liver tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 2 publications below |
Product Information
13237-1-AP targets DPYS in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DPYS fusion protein Ag3972 |
| Full Name | dihydropyrimidinase |
| Calculated Molecular Weight | 519 aa, 57 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC034395 |
| Gene Symbol | DPYS |
| Gene ID (NCBI) | 1807 |
| RRID | AB_2094329 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DPYS(Dihydropyrimidinase)also known as 5,6-dihydropyrimidine amidohydrolase, or DHP, is the second enzyme in uracil and thymine catabolism. However, in normal human extrahepatic tissues, DPYS, rather than dihydrouracil dehydrogenase, is the rate-limiting enzyme in the pyrimidine base catabolic pathway. DHPase in solid tumors is increased when compared with normal tissues(PMID: 8307005).Defects in DPYS are the cause of dihydropyrimidinase deficiency (DHPD).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DPYS antibody 13237-1-AP | Download protocol |
| IHC protocol for DPYS antibody 13237-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Dihydropyrimidinase protects from DNA replication stress caused by cytotoxic metabolites.
| ||
Cancer Cell Int Identification of crucial genes of pyrimidine metabolism as biomarkers for gastric cancer prognosis. | ||
Arthritis Res Ther Investigation of ferroptosis-associated molecular subtypes and immunological characteristics in lupus nephritis based on artificial neural network learning |
