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DPP8 Polyclonal antibody

DPP8 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12752-1-AP

Synonyms

dipeptidyl peptidase 8, Dipeptidyl peptidase VIII, DP8, DPP VIII, DPP8, DPRP 1, DPRP1, MSTP135, MSTP141, Prolyl dipeptidase DPP8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DPP8 Polyclonal antibody (12752-1-AP)

    WB analysis using 12752-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12752-1-AP (DPP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using DPP8 Polyclonal antibody (12752-1-AP)

    IP experiment of mouse testis using 12752-1-AP

    IP result of anti-DPP8 (IP:12752-1-AP, 4ug; Detection:12752-1-AP 1:500) with mouse testis tissue lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using DPP8 Polyclonal antibody (12752-1-AP)

    IHC staining of human prostate cancer using 12752-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12752-1-AP (DPP8 antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inhuman placenta tissue, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12752-1-AP targets DPP8 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DPP8 fusion protein Ag3262
Full Name dipeptidyl-peptidase 8
Calculated Molecular Weight 847 aa, 97 kDa
Observed Molecular Weight 75-83 kDa
GenBank Accession NumberBC030688
Gene Symbol DPP8
Gene ID (NCBI) 54878
RRIDAB_10638438
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DPP8 antibody 12752-1-APDownload protocol
IHC protocol for DPP8 antibody 12752-1-APDownload protocol
IP protocol for DPP8 antibody 12752-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols