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DPM1 Polyclonal antibody

DPM1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12403-2-AP

Synonyms

CDGIE, DPM synthase, DPM1, Mannose P dolichol synthase, MPD synthase, MPDS

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DPM1 Polyclonal antibody (12403-2-AP)

    WB analysis of HeLa using 12403-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12403-2-AP (DPM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using DPM1 Polyclonal antibody (12403-2-AP)

    WB analysis of HepG2 using 12403-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12403-2-AP (DPM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DPM1 Polyclonal antibody (12403-2-AP)

    IHC staining of human liver cancer using 12403-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12403-2-AP (DPM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DPM1 Polyclonal antibody (12403-2-AP)

    IHC staining of human liver cancer using 12403-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12403-2-AP (DPM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

12403-2-AP targets DPM1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DPM1 fusion protein Ag1128
Full Name dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit
Calculated Molecular Weight 260 aa, 30 kDa
Observed Molecular Weight 27-32 kDa
GenBank Accession NumberBC007073
Gene Symbol DPM1
Gene ID (NCBI) 8813
RRIDAB_2093270
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DPM1 antibody 12403-2-APDownload protocol
IHC protocol for DPM1 antibody 12403-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Genet

Identification of a Novel Glycosyltransferase Prognostic Signature in Hepatocellular Carcinoma Based on LASSO Algorithm.

Authors - Zhiyang Zhou
View Article
WB

FEBS Lett

Aspirin damages the cell wall of Saccharomyces cerevisiae by inhibiting the expression and activity of dolichol-phosphate mannose synthase 1.

Authors - Ming Li
View Article