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DPH2 Polyclonal antibody

DPH2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14866-1-AP

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DPH2 Polyclonal antibody (14866-1-AP)

    WB analysis of HeLa using 14866-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14866-1-AP (DPH2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14866-1-AP targets DPH2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DPH2 fusion protein Ag6678
Full Name DPH2 homolog (S. cerevisiae)
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC001389
Gene Symbol DPH2
Gene ID (NCBI) 1802
RRIDAB_2093254
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2). Mutants of the DPH1, DPH2, and DPH5 (611075) genes, involved in diphthamide biosynthesis, were identified as resistant to both anthrax toxin and diphtheria toxin. This antibody works well in WB, IP, IHC and IF application.

Protocols

Product Specific Protocols
WB protocol for DPH2 antibody 14866-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols