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DPH2 Polyclonal antibody

DPH2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12367-1-AP

Synonyms

S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase 2, HsDph2, DPH2L2, Diphtheria toxin resistance protein 2, 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using DPH2 Polyclonal antibody (12367-1-AP)

    WB analysis of human brain using 12367-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12367-1-AP (DPH2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DPH2 Polyclonal antibody (12367-1-AP)

    WB analysis using 12367-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12367-1-AP (DPH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using DPH2 Polyclonal antibody (12367-1-AP)

    WB analysis of PC-3 using 12367-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12367-1-AP (DPH2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using DPH2 Polyclonal antibody (12367-1-AP)

    IP experiment of mouse skeletal muscle using 12367-1-AP

    IP result of anti-DPH2 (IP:12367-1-AP, 3ug; Detection:12367-1-AP 1:1000) with mouse skeletal muscle tissue lysate 6000ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DPH2 Polyclonal antibody (12367-1-AP)

    IHC staining of human colon cancer using 12367-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12367-1-AP (DPH2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using DPH2 Polyclonal antibody (12367-1-AP)

    IF Staining of PC-3 using 12367-1-AP

    Immunofluorescent analysis of PC-3 cells using 12367-1-AP (DPH2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inCOLO 320 cells, human brain tissue, PC-3 cells
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12367-1-AP targets DPH2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DPH2 fusion protein Ag3042
Full Name DPH2 homolog (S. cerevisiae)
Calculated Molecular Weight 489 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC016956
Gene Symbol DPH2
Gene ID (NCBI) 1802
RRIDAB_2093256
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2). When overexpressed, suppresses colony formation ability and growth rate of ovarian cancer cells. Mutants of the DPH1, DPH2, and DPH5 (611075) genes, involved in diphthamide biosynthesis, were identified as resistant to both anthrax toxin and diphtheria toxin. This antibody works well in WB, IP, IHC and IF application.

Protocols

Product Specific Protocols
WB protocol for DPH2 antibody 12367-1-APDownload protocol
IHC protocol for DPH2 antibody 12367-1-APDownload protocol
IF protocol for DPH2 antibody 12367-1-APDownload protocol
IP protocol for DPH2 antibody 12367-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols