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DOK2 Polyclonal antibody

DOK2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14087-1-AP

Synonyms

Docking protein 2, docking protein 2, 56kDa, DOK2, p56(dok 2), p56DOK, p56dok 2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Jurkat cells using DOK2 Polyclonal antibody (14087-1-AP)

    WB analysis of Jurkat using 14087-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14087-1-AP (DOK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using DOK2 Polyclonal antibody (14087-1-AP)

    IHC staining of human lymphoma using 14087-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 14087-1-AP (DOK2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using DOK2 Polyclonal antibody (14087-1-AP)

    IHC staining of human lymphoma using 14087-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 14087-1-AP (DOK2 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

14087-1-AP targets DOK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DOK2 fusion protein Ag5218
Full Name docking protein 2, 56kDa
Calculated Molecular Weight 412 aa, 45 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC032623
Gene Symbol DOK2
Gene ID (NCBI) 9046
RRIDAB_2230792
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Docking protein 2 (Dok2) is an adapter protein which is involved in hematopoiesis. Dok2 is able to control Klf1 expression by transcriptional regulation through directly binding to its promoter region (PMID: 25075100).

Protocols

Product Specific Protocols
WB protocol for DOK2 antibody 14087-1-APDownload protocol
IHC protocol for DOK2 antibody 14087-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Histopathology

Expression of T-cell receptor signalling pathway components in extranodal NK/T-cell lymphoma.

Authors - Tomoko Miyata-Takata
View Article