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DOK1 Polyclonal antibody

DOK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17822-1-AP

Synonyms

pp62, P62DOK, Docking protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using DOK1 Polyclonal antibody (17822-1-AP)

    WB analysis of K-562 using 17822-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17822-1-AP (DOK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using DOK1 Polyclonal antibody (17822-1-AP)

    WB analysis of K-562 using 17822-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17822-1-AP (DOK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using DOK1 Polyclonal antibody (17822-1-AP)

    IHC staining of human ovary cancer using 17822-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17822-1-AP (DOK1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inK-562 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17822-1-AP targets DOK1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DOK1 fusion protein Ag12189
Full Name docking protein 1, 62kDa (downstream of tyrosine kinase 1)
Calculated Molecular Weight 48 aa, 52 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC111055
Gene Symbol DOK1
Gene ID (NCBI) 1796
RRIDAB_2274420
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The downstream of tyrosine kinase 1gene (DOK1) has emerged as a newly identified tumor suppressor gene that encodes a multi-domain adapter protein and acts as a negative regulator of signaling pathways involved in several cellular functions. DOK1 inhibits cell proliferation, down regulates MAP kinase activity, and has an opposing role in leukemogenesis and promotes cell spreading, motility, and apoptosis. (PMID: 24809689)

Protocols

Product Specific Protocols
WB protocol for DOK1 antibody 17822-1-APDownload protocol
IHC protocol for DOK1 antibody 17822-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols