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DOCK9 Polyclonal antibody

DOCK9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 18987-1-AP

Synonyms

Zizimin-1, ZIZ1, dedicator of cytokinesis 9, Cdc42 guanine nucleotide exchange factor zizimin-1

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using DOCK9 Polyclonal antibody (18987-1-AP)

    WB analysis of A549 using 18987-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DOCK9 Polyclonal antibody (18987-1-AP)

    WB analysis using 18987-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using DOCK9 Polyclonal antibody (18987-1-AP)

    WB analysis of human brain using 18987-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using DOCK9 Polyclonal antibody (18987-1-AP)

    WB analysis of human heart using 18987-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using DOCK9 Polyclonal antibody (18987-1-AP)

    WB analysis of human placenta using 18987-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using DOCK9 Polyclonal antibody (18987-1-AP)

    WB analysis of mouse brain using 18987-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using DOCK9 Polyclonal antibody (18987-1-AP)

    IP experiment of mouse brain using 18987-1-AP

    IP result of anti-DOCK9 (IP:18987-1-AP, 4ug; Detection:18987-1-AP 1:800) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using DOCK9 Polyclonal antibody (18987-1-AP)

    IHC staining of human kidney using 18987-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18987-1-AP (DOCK9 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using DOCK9 Polyclonal antibody (18987-1-AP)

    IHC staining of human kidney using 18987-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18987-1-AP (DOCK9 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using DOCK9 Polyclonal antibody (18987-1-AP)

    IHC staining of human heart using 18987-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18987-1-AP (DOCK9 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using DOCK9 Polyclonal antibody (18987-1-AP)

    IHC staining of human heart using 18987-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18987-1-AP (DOCK9 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DOCK9 Polyclonal antibody (18987-1-AP)

    IF Staining of HeLa using 18987-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DOCK9 antibody (18987-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inA549 cells, human brain tissue, mouse brain tissue, human heart tissue, human placenta tissue, HeLa cells, MCF-7 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

18987-1-AP targets DOCK9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name dedicator of cytokinesis 9
Calculated Molecular Weight 239 kDa
Observed Molecular Weight 200-236 kDa
GenBank Accession NumberNM_015296
Gene Symbol DOCK9
Gene ID (NCBI) 23348
RRIDAB_10596777
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DOCK9 is a guanine nucleotide exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Its overexpression induces filopodia formation.

Protocols

Product Specific Protocols
WB protocol for DOCK9 antibody 18987-1-APDownload protocol
IHC protocol for DOCK9 antibody 18987-1-APDownload protocol
IF protocol for DOCK9 antibody 18987-1-APDownload protocol
IP protocol for DOCK9 antibody 18987-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Commun Biol

Brain endothelial cells promote breast cancer cell extravasation to the brain via EGFR-DOCK4-RAC1 signalling

Authors - Chiara Galloni
View Article