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DOCK6 Polyclonal antibody

DOCK6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25087-1-AP

Synonyms

dedicator of cytokinesis 6, DOCK6, KIAA1395, ZIR1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DOCK6 Polyclonal antibody (25087-1-AP)

    WB analysis using 25087-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 25087-1-AP (DOCK6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using DOCK6 Polyclonal antibody (25087-1-AP)

    IHC staining of human colon using 25087-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25087-1-AP (DOCK6 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using DOCK6 Polyclonal antibody (25087-1-AP)

    IHC staining of human colon using 25087-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25087-1-AP (DOCK6 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DOCK6 Polyclonal antibody (25087-1-AP)

    IHC staining of human colon cancer using 25087-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25087-1-AP (DOCK6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DOCK6 Polyclonal antibody (25087-1-AP)

    IHC staining of human colon cancer using 25087-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25087-1-AP (DOCK6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inCOLO 320 cells
Positive IHC detected inhuman colon tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25087-1-AP is DOCK6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DOCK6 fusion protein Ag18901
Full Name dedicator of cytokinesis 6
Calculated Molecular Weight 2047 aa, 230 kDa
Observed Molecular Weight 230 kDa
GenBank Accession NumberBC146786
Gene Symbol DOCK6
Gene ID (NCBI) 57572
RRIDAB_2879889
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DOCK6 antibody 25087-1-APDownload protocol
IHC protocol for DOCK6 antibody 25087-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Cell Biol

Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.

Authors - Li Wang
View Article
humanWB

BMC Med Genomics

Attenuated clinical and osteoclastic phenotypes of Paget's disease of bone linked to the p.Pro392Leu/SQSTM1 mutation by a rare variant in the DOCK6 gene.

Authors - Mariam Dessay
View Article