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DOCK4 Polyclonal antibody

DOCK4 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27151-1-AP

Synonyms

dedicator of cytokinesis 4, DOCK4, KIAA0716

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293T cells using DOCK4 Polyclonal antibody (27151-1-AP)

    WB analysis of HEK-293T using 27151-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 27151-1-AP (DOCK4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DOCK4 Polyclonal antibody (27151-1-AP)

    IF Staining of HeLa using 27151-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 27151-1-AP (DOCK4 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293T cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27151-1-AP is DOCK4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DOCK4 fusion protein Ag25946
Full Name dedicator of cytokinesis 4
Calculated Molecular Weight 2011 aa, 230 kDa
Observed Molecular Weight 225 kDa
GenBank Accession NumberBC117689
Gene Symbol DOCK4
Gene ID (NCBI) 9732
RRIDAB_2880776
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DOCK4 antibody 27151-1-APDownload protocol
IF protocol for DOCK4 antibody 27151-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols