• Featured Product
  • KD/KO Validated

DOC2B Polyclonal antibody

DOC2B Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 20574-1-AP

Synonyms

Doc2 beta, DOC2B, DOC2BL, double C2 like domains, beta



Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, mouse kidney tissue
Positive IF/ICC detected inHepG2 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20574-1-AP targets DOC2B in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name double C2-like domains, beta
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberNM_003585
Gene Symbol DOC2B
Gene ID (NCBI) 8447
RRIDAB_10696316
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DOC2B may be involved in constitutive membrane trafficking including dynein-dependent intracellular vesicle transport. In vitro, binds calcium and phospholipids. The antibody is specific to DOC2B.

Protocols

Product Specific Protocols
WB protocol for DOC2B antibody 20574-1-APDownload protocol
IF protocol for DOC2B antibody 20574-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Diabetes

Doc2b Protects β-Cells Against Inflammatory Damage and Enhances Function.

Authors - Arianne Aslamy
  • KO Validated
humanWB

Cell Biol Toxicol

Metastatic suppression by DOC2B is mediated by inhibition of epithelial-mesenchymal transition and induction of senescence.

Authors - Samatha Bhat
  • KD Validated
humanWB,CoIP

Pharmacol Res

DOC2B is a negative regulator of Wnt/β-catenin signaling pathway in cervical cancer.

Authors - Divya Adiga
  • KD Validated
humanWB

J Biol Chem

DNA Promoter Methylation Dependent Transcription of Double C2 Like Domain Beta (DOC2B) Gene Regulates Tumor Growth in Human Cervical Cancer.

Authors - Shama Prasada Kabekkodu
humanWB,IF

Free Radic Biol Med

Double C-2 like domain beta (DOC2B) induces calcium dependent oxidative stress to promote lipotoxicity and mitochondrial dysfunction for its tumor suppressive function

Authors - Divya Adiga
mouse,ratWB

Int J Mol Sci

A Novel Role for DOC2B in Ameliorating Palmitate-Induced Glucose Uptake Dysfunction in Skeletal Muscle Cells via a Mechanism Involving β-AR Agonism and Cofilin

Authors - Jinhee Hwang