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DOC2B Polyclonal antibody
DOC2B Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 20574-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, mouse brain tissue, mouse kidney tissue |
Positive IF/ICC detected in | HepG2 cells, Hela cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 7 publications below |
IF | See 3 publications below |
CoIP | See 1 publications below |
Product Information
20574-1-AP targets DOC2B in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | double C2-like domains, beta |
Calculated Molecular Weight | 46 kDa |
Observed Molecular Weight | 46 kDa |
GenBank Accession Number | NM_003585 |
Gene Symbol | DOC2B |
Gene ID (NCBI) | 8447 |
RRID | AB_10696316 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DOC2B may be involved in constitutive membrane trafficking including dynein-dependent intracellular vesicle transport. In vitro, binds calcium and phospholipids. The antibody is specific to DOC2B.
Protocols
Product Specific Protocols | |
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WB protocol for DOC2B antibody 20574-1-AP | Download protocol |
IF protocol for DOC2B antibody 20574-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Diabetes Doc2b Protects β-Cells Against Inflammatory Damage and Enhances Function.
| ||
Cell Biol Toxicol Metastatic suppression by DOC2B is mediated by inhibition of epithelial-mesenchymal transition and induction of senescence.
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Pharmacol Res DOC2B is a negative regulator of Wnt/β-catenin signaling pathway in cervical cancer.
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J Biol Chem DNA Promoter Methylation Dependent Transcription of Double C2 Like Domain Beta (DOC2B) Gene Regulates Tumor Growth in Human Cervical Cancer. | ||
Free Radic Biol Med Double C-2 like domain beta (DOC2B) induces calcium dependent oxidative stress to promote lipotoxicity and mitochondrial dysfunction for its tumor suppressive function | ||
Int J Mol Sci A Novel Role for DOC2B in Ameliorating Palmitate-Induced Glucose Uptake Dysfunction in Skeletal Muscle Cells via a Mechanism Involving β-AR Agonism and Cofilin |