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DNTTIP1 Polyclonal antibody

DNTTIP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11637-1-AP

Synonyms

Terminal deoxynucleotidyltransferase-interacting factor 1, TdT-interacting factor 1, TdIF1, DNTTIP 1, Deoxynucleotidyltransferase terminal-interacting protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DNTTIP1 Polyclonal antibody (11637-1-AP)

    WB analysis of HeLa cells using 11637-1-AP

    HeLa cell were subjected to SDS PAGE followed by western blot with 11637-1-AP (DNTTIP1 Antibody) at dilution of 1:500.

  • Immunoprecipitation (IP) experiment of HeLa cells using DNTTIP1 Polyclonal antibody (11637-1-AP)

    IP experiment of HeLa using 11637-1-AP

    IP result of anti-DNTTIP1 (IP:11637-1-AP, 4ug; Detection:11637-1-AP 1:300) with HeLa cells lysate 1200ug.

  • Immunoprecipitation (IP) experiment of HeLa cells using DNTTIP1 Polyclonal antibody (11637-1-AP)

    IP experiment of HeLa using 11637-1-AP

    IP result of anti-DNTTIP1 (IP:11637-1-AP, 4ug; Detection:11637-1-AP 1:300) with HeLa cells lysate 1200ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DNTTIP1 Polyclonal antibody (11637-1-AP)

    IF Staining of HeLa using 11637-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11637-1-AP (DNTTIP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11637-1-AP targets DNTTIP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNTTIP1 fusion protein Ag2213
Full Name deoxynucleotidyltransferase, terminal, interacting protein 1
Calculated Molecular Weight 329 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC024290
Gene Symbol DNTTIP1
Gene ID (NCBI) 116092
RRIDAB_2877787
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNTTIP1, also named as Deoxynucleotidyltransferase terminal-interacting protein 1, is a 329 amino acid protein ,which contains a Nuclear localization signal and localizes in the nuclear. DNTTIP1 also acts as a transcriptional regulator, which binding to the consensus sequence 5'-GNTGCATG-3' following an AT-tract. Associates with RAB20 promoter and positively regulates its transcription.

Protocols

Product Specific Protocols
IF protocol for DNTTIP1 antibody 11637-1-APDownload protocol
IP protocol for DNTTIP1 antibody 11637-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols