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DNPEP Polyclonal antibody

DNPEP Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15016-1-AP

Synonyms

EC:3.4.11.21, DAP, ASPEP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNPEP Polyclonal antibody (15016-1-AP)

    WB analysis using 15016-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15016-1-AP (DNPEP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using DNPEP Polyclonal antibody (15016-1-AP)

    WB analysis of MCF-7 using 15016-1-AP

    WB result of DNPEP antibody (15016-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNPEP transfected MCF-7 cells.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using DNPEP Polyclonal antibody (15016-1-AP)

    IP experiment of HEK-293 using 15016-1-AP

    IP result of anti-DNPEP (IP:15016-1-AP, 3ug; Detection:15016-1-AP 1:500) with HEK-293 cells lysate 1760ug.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using DNPEP Polyclonal antibody (15016-1-AP)

    IF Staining of MCF-7 using 15016-1-AP

    Immunofluorescent analysis of MCF-7 cells using 15016-1-AP (DNPEP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells, K-562 cells
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15016-1-AP targets DNPEP in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNPEP fusion protein Ag6966
Full Name aspartyl aminopeptidase
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC000653
Gene Symbol DNPEP
Gene ID (NCBI) 23549
RRIDAB_2094293
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNPEP, also named as ASPEP and DAP, plays an important role in intracellular protein and peptide metabolism. It catalyzes the hydrolysis of N-terminal aspartyl and glutamyl residues.

Protocols

Product Specific Protocols
WB protocol for DNPEP antibody 15016-1-APDownload protocol
IF protocol for DNPEP antibody 15016-1-APDownload protocol
IP protocol for DNPEP antibody 15016-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell Biol

Chondrocyte-specific microRNA-140 regulates endochondral bone development and targets Dnpep to modulate bone morphogenetic protein signaling.

Authors - Nakamura Yukio Y
View Article