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- KD/KO Validated
DNALI1 Polyclonal antibody
DNALI1 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 17601-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse testis tissue, rat testis tissue |
Positive IP detected in | mouse testis tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 7 publications below |
IF | See 7 publications below |
CoIP | See 1 publications below |
Product Information
17601-1-AP targets DNALI1 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | DNALI1 fusion protein Ag11652 |
Full Name | dynein, axonemal, light intermediate chain 1 |
Calculated Molecular Weight | 258 aa, 30 kDa |
Observed Molecular Weight | 30-33 kDa |
GenBank Accession Number | BC046117 |
Gene Symbol | DNALI1 |
Gene ID (NCBI) | 7802 |
RRID | AB_2095372 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DNALI1 belongs to the inner dynein arm light chain family and encodes a subunit of the light intermediate chain of inner dynein arms of the axoneme of sperm flagella (PMID: 36792588). DNALI1 promotes neurodegeneration after traumatic brain injury via inhibition of autophagosome-lysosome fusion (PMID: 38348540). Mutation in DNALI1 Causes Male Infertility with Severe Oligoasthenoteratozoospermia in Humans (PMID: 38212584).
Protocols
Product Specific Protocols | |
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WB protocol for DNALI1 antibody 17601-1-AP | Download protocol |
IP protocol for DNALI1 antibody 17601-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Adv Sci (Weinh) DNALI1 Promotes Neurodegeneration after Traumatic Brain Injury via Inhibition of Autophagosome-Lysosome Fusion | ||
Front Cell Dev Biol PIH1D3-knockout rats exhibit full ciliopathy features and dysfunctional pre-assembly and loading of dynein arms in motile cilia | ||
Development CCDC183 is essential for cytoplasmic invagination around the flagellum during spermiogenesis and male fertility | ||
Clin Genet DNAH2 is a novel candidate gene associated with multiple morphological abnormalities of the sperm flagella. | ||
Exp Anim The motor domain of testis-enriched kinesin KIF9 is essential for its localization in the mouse flagellum. |