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DNALI1 Polyclonal antibody

DNALI1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17601-1-AP

Synonyms

DNALI1, hp28, P28



Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17601-1-AP targets DNALI1 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNALI1 fusion protein Ag11652
Full Name dynein, axonemal, light intermediate chain 1
Calculated Molecular Weight 258 aa, 30 kDa
Observed Molecular Weight 30-33 kDa
GenBank Accession NumberBC046117
Gene Symbol DNALI1
Gene ID (NCBI) 7802
RRIDAB_2095372
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNALI1 belongs to the inner dynein arm light chain family and encodes a subunit of the light intermediate chain of inner dynein arms of the axoneme of sperm flagella (PMID: 36792588). DNALI1 promotes neurodegeneration after traumatic brain injury via inhibition of autophagosome-lysosome fusion (PMID: 38348540). Mutation in DNALI1 Causes Male Infertility with Severe Oligoasthenoteratozoospermia in Humans (PMID: 38212584).

Protocols

Product Specific Protocols
WB protocol for DNALI1 antibody 17601-1-APDownload protocol
IP protocol for DNALI1 antibody 17601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Adv Sci (Weinh)

DNALI1 Promotes Neurodegeneration after Traumatic Brain Injury via Inhibition of Autophagosome-Lysosome Fusion

Authors - Xulong Ding
ratWB,IF

Front Cell Dev Biol

PIH1D3-knockout rats exhibit full ciliopathy features and dysfunctional pre-assembly and loading of dynein arms in motile cilia

Authors - Tingting Zhang
mouseWB

Development

CCDC183 is essential for cytoplasmic invagination around the flagellum during spermiogenesis and male fertility

Authors - Keisuke Shimada
humanIF

Clin Genet

DNAH2 is a novel candidate gene associated with multiple morphological abnormalities of the sperm flagella.

Authors - Yang Li
mouseWB,IF

Basic Clin Androl

Dnali1 is required for sperm motility and male fertility in mice

Authors - Yiling Zhou
mouseWB

Exp Anim

The motor domain of testis-enriched kinesin KIF9 is essential for its localization in the mouse flagellum.

Authors - Haruhiko Miyata