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DNAL1 Polyclonal antibody

DNAL1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 15809-1-AP

Synonyms

C14orf168, DNAL1, Dynein light chain 1, axonemal

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNAL1 Polyclonal antibody (15809-1-AP)

    WB analysis using 15809-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15809-1-AP (DNAL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DNAL1 Polyclonal antibody (15809-1-AP)

    WB analysis of HeLa using 15809-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15809-1-AP (DNAL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using DNAL1 Polyclonal antibody (15809-1-AP)

    WB analysis of mouse brain using 15809-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15809-1-AP (DNAL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using DNAL1 Polyclonal antibody (15809-1-AP)

    WB analysis of human brain using 15809-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15809-1-AP (DNAL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using DNAL1 Polyclonal antibody (15809-1-AP)

    IP experiment of HeLa using 15809-1-AP

    IP result of anti-DNAL1 (IP:15809-1-AP, 4ug; Detection:15809-1-AP 1:500) with HeLa cells lysate 2600 ug.

Tested Applications

Positive WB detected inHeLa cells, human brain tissue, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15809-1-AP targets DNAL1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAL1 fusion protein Ag8545
Full Name dynein, axonemal, light chain 1
Calculated Molecular Weight 151 aa, 17 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC005343
Gene Symbol DNAL1
Gene ID (NCBI) 83544
RRIDAB_2095365
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNAL1 was originally documented as the Chlamydomonas outer arm dynein axonemal light chain 1. DNAL1 has been documented to link components of the cytoskeleton with the molecular motor dynein, perhaps acting as a scaffold for larger functional structures (PMID: 22018492).

Protocols

Product Specific Protocols
WB protocol for DNAL1 antibody 15809-1-APDownload protocol
IP protocol for DNAL1 antibody 15809-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols