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  • KD/KO Validated

DNAJC7 Polyclonal antibody

DNAJC7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11090-1-AP

Synonyms

TTC2, TPR2, DNAJC 7, DANJC7, CCRP



Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, Jurkat cells, moust testis
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11090-1-AP targets DNAJC7 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJC7 fusion protein Ag1572
Full Name DnaJ (Hsp40) homolog, subfamily C, member 7
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC011837
Gene Symbol DNAJC7
Gene ID (NCBI) 7266
RRIDAB_2293179
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DnaJC7, otherwise referred to as Tpr2 or CCRP, is a conserved, type of J protein that is highly expressed within the brain. DnaJC7 has been found to modulate the flow of substrates from Hsp70 to Hsp90, providing a control mechanism in proteins such as glucocorticoids and progesterone receptor.

Protocols

Product Specific Protocols
WB protocol for DNAJC7 antibody 11090-1-APDownload protocol
IHC protocol for DNAJC7 antibody 11090-1-APDownload protocol
IF protocol for DNAJC7 antibody 11090-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseCoIP

Nat Commun

DnaJC7 binds natively folded structural elements in tau to inhibit amyloid formation.

Authors - Zhiqiang Hou
humanWB,IF

Biochem Pharmacol

Indirect activation of constitutive androstane receptor in three-dimensionally cultured HepG2 cells.

Authors - Kosuke Yokobori
  • KO Validated
humanWB

Biochem Biophys Res Commun

Co-chaperon DnaJC7/TPR2 enhances p53 stability and activity through blocking the complex formation between p53 and MDM2.

Authors - Kubo Natsumi N
humanWB

bioRxiv

DnaJC7 specifically regulates tau seeding

Authors - Valerie A Perez
  • KO Validated
humanWB

Elife

DnaJC7 specifically regulates tau seeding

Authors - Valerie Ann Perez
humanWB

Cancers (Basel)

Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin

Authors - Mohamed Alalem