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DNAJC7 Polyclonal antibody
DNAJC7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 11090-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, HEK-293 cells, Jurkat cells, moust testis |
Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 6 publications below |
IF | See 1 publications below |
CoIP | See 1 publications below |
Product Information
11090-1-AP targets DNAJC7 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | DNAJC7 fusion protein Ag1572 |
Full Name | DnaJ (Hsp40) homolog, subfamily C, member 7 |
Calculated Molecular Weight | 56 kDa |
Observed Molecular Weight | 56 kDa |
GenBank Accession Number | BC011837 |
Gene Symbol | DNAJC7 |
Gene ID (NCBI) | 7266 |
RRID | AB_2293179 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DnaJC7, otherwise referred to as Tpr2 or CCRP, is a conserved, type of J protein that is highly expressed within the brain. DnaJC7 has been found to modulate the flow of substrates from Hsp70 to Hsp90, providing a control mechanism in proteins such as glucocorticoids and progesterone receptor.
Protocols
Product Specific Protocols | |
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WB protocol for DNAJC7 antibody 11090-1-AP | Download protocol |
IHC protocol for DNAJC7 antibody 11090-1-AP | Download protocol |
IF protocol for DNAJC7 antibody 11090-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun DnaJC7 binds natively folded structural elements in tau to inhibit amyloid formation. | ||
Biochem Pharmacol Indirect activation of constitutive androstane receptor in three-dimensionally cultured HepG2 cells.
| ||
Biochem Biophys Res Commun Co-chaperon DnaJC7/TPR2 enhances p53 stability and activity through blocking the complex formation between p53 and MDM2. | ||
Cancers (Basel) Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin |