WB results of DNAJC21 antibody (23411-1-AP, 1:2000) with si-control and si-DNAJC21 transfected HEK293 cells.
WB analysis using 23411-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 23411-1-AP (DNAJC21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 23411-1-AP (DNAJC21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 23411-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 23411-1-AP (DNAJC21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
rat brain tissue were subjected to SDS PAGE followed by western blot with 23411-1-AP (DNAJC21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 23411-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 23411-1-AP (DNAJC21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 23411-1-AP (DNAJC21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 23411-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 23411-1-AP (DNAJC21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 23411-1-AP (DNAJC21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 23411-1-AP
IP result of anti-DNAJC21 (IP:23411-1-AP, 4ug; Detection:23411-1-AP 1:1000) with HEK-293 cells lysate 2700ug.
IP result of anti-DNAJC21 (IP:23411-1-AP, 4ug; Detection:23411-1-AP 1:1000) with HEK-293 cells lysate 2700ug.
IHC staining of human pancreas using 23411-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 23411-1-AP (DNAJC21 Antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 23411-1-AP (DNAJC21 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 23411-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 23411-1-AP (DNAJC21 Antibody) at dilution of 1:50 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 23411-1-AP (DNAJC21 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 23411-1-AP
Immunofluorescent analysis of HeLa cells using 23411-1-AP (DNAJC21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of HeLa cells using 23411-1-AP (DNAJC21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
HEK-293 cells, mouse brain tissue, HeLa cells, rat brain tissue
Positive IP detected in
HEK-293 cells
Positive IHC detected in
human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:10000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
DNAJC21 is a ubiquitously expressed DnaJ (heat shock protein 40) chaperone protein. Recently DNAJC21 was reported to be implicated in rRNA biogenesis. Loss of DNAJC21 induced abnormal cell morphology and inhibited cell growth. Mutations in DNAJC21 cause bone marrow failure syndrome. (27346687)
WB results of DNAJC21 antibody (23411-1-AP, 1:2000) with si-control and si-DNAJC21 transfected HEK293 cells.
WB analysis using 23411-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 23411-1-AP (DNAJC21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 23411-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 23411-1-AP (DNAJC21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 23411-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 23411-1-AP (DNAJC21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 23411-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 23411-1-AP (DNAJC21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human pancreas using 23411-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 23411-1-AP (DNAJC21 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 23411-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 23411-1-AP (DNAJC21 Antibody) at dilution of 1:50 (under 40x lens).
IP Figures
IP experiment of HEK-293 using 23411-1-AP
IP result of anti-DNAJC21 (IP:23411-1-AP, 4ug; Detection:23411-1-AP 1:1000) with HEK-293 cells lysate 2700ug.
IF/ICC Figures
IF Staining of HeLa using 23411-1-AP
Immunofluorescent analysis of HeLa cells using 23411-1-AP (DNAJC21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
DNAJC21 Polyclonal antibody
Catalog Number
23411-1-AP
Citations
2
Dilutions
WB : 1:2000-1:10000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:20-1:200
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
