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DNAJC15 Polyclonal antibody

DNAJC15 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16063-1-AP

Synonyms

DNAJC15, DNAJD1, HSD18, MCJ

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using DNAJC15 Polyclonal antibody (16063-1-AP)

    WB analysis of mouse liver using 16063-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16063-1-AP (DNAJC15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using DNAJC15 Polyclonal antibody (16063-1-AP)

    WB analysis of human brain using 16063-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16063-1-AP (DNAJC15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using DNAJC15 Polyclonal antibody (16063-1-AP)

    IHC staining of human liver using 16063-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16063-1-AP (DNAJC15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using DNAJC15 Polyclonal antibody (16063-1-AP)

    IHC staining of human liver using 16063-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16063-1-AP (DNAJC15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using DNAJC15 Polyclonal antibody (16063-1-AP)

    IHC staining of human liver using 16063-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16063-1-AP (DNAJC15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using DNAJC15 Polyclonal antibody (16063-1-AP)

    IHC staining of human liver using 16063-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16063-1-AP (DNAJC15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse liver tissue, human brain tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16063-1-AP targets DNAJC15 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJC15 fusion protein Ag8911
Full Name DnaJ (Hsp40) homolog, subfamily C, member 15
Calculated Molecular Weight 150 aa, 16 kDa
Observed Molecular Weight 18-20 kDa
GenBank Accession NumberBC010910
Gene Symbol DNAJC15
Gene ID (NCBI) 29103
RRIDAB_10598307
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DNAJC15 antibody 16063-1-APDownload protocol
IHC protocol for DNAJC15 antibody 16063-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancers (Basel)

Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin

Authors - Mohamed Alalem
View Article