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DNAJC12 Polyclonal antibody
DNAJC12 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 12338-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, BxPC-3 cells, HEK-293 cells |
Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 3 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
12338-1-AP targets DNAJC12 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | DNAJC12 fusion protein Ag2989 |
Full Name | DnaJ (Hsp40) homolog, subfamily C, member 12 |
Calculated Molecular Weight | 198 aa, 23 kDa |
Observed Molecular Weight | 28 kDa |
GenBank Accession Number | BC017018 |
Gene Symbol | DNAJC12 |
Gene ID (NCBI) | 56521 |
RRID | AB_2246188 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for DNAJC12 antibody 12338-1-AP | Download protocol |
IHC protocol for DNAJC12 antibody 12338-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Rep Med Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application | ||
Cell Stress Chaperones The co-chaperone DNAJC12 binds to Hsc70 and is upregulated by endoplasmic reticulum stress. | ||
Ann Surg Oncol Increased Expression of DNAJC12 is Associated with Aggressive Phenotype of Gastric Cancer.
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