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DNAJB8 Polyclonal antibody

DNAJB8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 17071-1-AP

Synonyms

DJ6

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DNAJB8 Polyclonal antibody (17071-1-AP)

    WB analysis of HeLa using 17071-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17071-1-AP (DNAJB8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using DNAJB8 Polyclonal antibody (17071-1-AP)

    WB analysis of human brain using 17071-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17071-1-AP (DNAJB8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17071-1-AP targets DNAJB8 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJB8 fusion protein Ag10899
Full Name DnaJ (Hsp40) homolog, subfamily B, member 8
Calculated Molecular Weight 232 aa, 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC029521
Gene Symbol DNAJB8
Gene ID (NCBI) 165721
RRIDAB_2094743
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNAJB8, a member of the DNAJ/HSP40 family of proteins, plays a crucial role in regulating chaperone HSP70 activity. It is known for its ability to suppress the aggregation of polyglutamine proteins through its C-terminal tail. Furthermore, DNAJB8 has been shown to inhibit TP53 through ubiquitin degradation in human colon cancer by directly interacting and upregulating MDR1, leading to increased oxaliplatin resistance. High levels of DNAJB8 in both colon tissues and serum are correlated with worse overall survival of colon cancer patients, suggesting that DNAJB8 level in serum sEV may serve as a potential biomarker for colon cancer and a promising therapeutic target for oxaliplatin-resistant cancer.

Protocols

Product Specific Protocols
WB protocol for DNAJB8 antibody 17071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

iScience

Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis.

Authors - Qianxing Zou
View Article
mouseWB,IF

STAR Protoc

Protocol for isolation and proteostatic analysis of sub-populations of spermatogenic cells in mouse.

Authors - Qianxing Zou
View Article
pigWB

Vet Microbiol

The heat shock protein DNAJB8 inhibits pseudorabies virus replication by autophagy

Authors - Xiaoyong Chen
View Article