rat heart tissue were subjected to SDS PAGE followed by western blot with 16453-1-AP (DNAJB5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
rat heart tissue were subjected to SDS PAGE followed by western blot with 16453-1-AP (DNAJB5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16453-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16453-1-AP (DNAJB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 16453-1-AP (DNAJB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16453-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16453-1-AP (DNAJB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
HepG2 cells were subjected to SDS PAGE followed by western blot with 16453-1-AP (DNAJB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 16453-1-AP
IP result of anti-DNAJB5 (IP:16453-1-AP, 4ug; Detection:16453-1-AP 1:500) with HepG2 cells lysate 3300 ug.
Immunohistochemical analysis of paraffin-embedded human testis using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 16453-1-AP
Immunofluorescent analysis of HepG2 cells using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of HepG2 cells using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human heart tissue, human kidney tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:2000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
16453-1-AP targets DNAJB5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
DNAJB5, also named as Hsc40, is an Hsp40 Family Protein with MW 39-40 kDa. It mediates antihypertrophic effects of Thioredoxin1 in the heart. This protein has some isoforms with MW 39 kDa and 47-51 kDa.
rat heart tissue were subjected to SDS PAGE followed by western blot with 16453-1-AP (DNAJB5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16453-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16453-1-AP (DNAJB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16453-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16453-1-AP (DNAJB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human heart using 16453-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 16453-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 16453-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 16453-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 16453-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 16453-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 40x lens).
IP Figures
IP experiment of HepG2 using 16453-1-AP
IP result of anti-DNAJB5 (IP:16453-1-AP, 4ug; Detection:16453-1-AP 1:500) with HepG2 cells lysate 3300 ug.
IF/ICC Figures
IF Staining of HepG2 using 16453-1-AP
Immunofluorescent analysis of HepG2 cells using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
DNAJB5 Polyclonal antibody
Catalog Number
16453-1-AP
Citations
-
Dilutions
WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:20-1:200
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
