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Tested Applications
| Positive WB detected in | HeLa cells, mouse serum, mouse skeletal muscle, rat skeletal muscle |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 10 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
13064-1-AP targets DNAJB4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DNAJB4 fusion protein Ag3756 Predict reactive species |
| Full Name | DnaJ (Hsp40) homolog, subfamily B, member 4 |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 38 kDa |
| GenBank Accession Number | BC034721 |
| Gene Symbol | DNAJB4 |
| Gene ID (NCBI) | 11080 |
| RRID | AB_2094422 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UDY4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DNAJB4 antibody 13064-1-AP | Download protocol |
| IHC protocol for DNAJB4 antibody 13064-1-AP | Download protocol |
| IF protocol for DNAJB4 antibody 13064-1-AP | Download protocol |
| IP protocol for DNAJB4 antibody 13064-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Neuropathol Distinctive chaperonopathy in skeletal muscle associated with the dominant variant in DNAJB4 | ||
Int J Mol Sci Genetic Deletion of HLJ1 Does Not Affect Blood Coagulation in Mice.
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Int J Clin Exp Pathol HLJ1 is a novel biomarker for colorectal carcinoma progression and overall patient survival. | ||
Elife HLJ1 amplifies endotoxin-induced sepsis severity by promoting IL-12 heterodimerization in macrophages
| ||
Acta Neuropathol Loss of function variants in DNAJB4 cause a myopathy with early respiratory failure
|
