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- KD/KO Validated
DNAJB12 Polyclonal antibody
DNAJB12 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF, IP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 16780-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, A549 cells, human brain tissue, human kidney tissue, human liver tissue, MCF-7 cells, MKN-45 cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human stomach tissue, human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| IF | See 3 publications below |
| IP | See 3 publications below |
| ChIP | See 1 publications below |
Product Information
16780-1-AP targets DNAJB12 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DNAJB12 fusion protein Ag10193 |
| Full Name | DnaJ (Hsp40) homolog, subfamily B, member 12 |
| Calculated Molecular Weight | 375 aa, 42 kDa |
| Observed Molecular Weight | 40-42 kDa |
| GenBank Accession Number | BC011812 |
| Gene Symbol | DNAJB12 |
| Gene ID (NCBI) | 54788 |
| RRID | AB_2094404 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DNAJB12 (JB12), a ER transmembrane chaperone, specifically interacts with Hsc70 to facilitate ER retention and delivery of membrane proteins for folding or degradation in the ERAD pathway. Hsc70 chaperone activities require the substrate targeting function of DNAJB12. DNAJB12 recruits specific substrates to contact Hsc70 and then stimulates its ATPase activity. (PMID: 34668642)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DNAJB12 antibody 16780-1-AP | Download protocol |
| IHC protocol for DNAJB12 antibody 16780-1-AP | Download protocol |
| IP protocol for DNAJB12 antibody 16780-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell An oncogenic JMJD6-DGAT1 axis tunes the epigenetic regulation of lipid droplet formation in clear cell renal cell carcinoma. | ||
Nat Commun The methyltransferase METTL9 mediates pervasive 1-methylhistidine modification in mammalian proteomes. | ||
J Cell Biol Reticulon protects the integrity of the ER membrane during ER escape of large macromolecular protein complexes. | ||
PLoS Pathog A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum. | ||
J Cell Biol Reticulons promote formation of ER-derived double-membrane vesicles that facilitate SARS-CoV-2 replication | ||
J Virol The ER membrane J protein C18 executes a distinct role in promoting SV40 membrane penetration. |
