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  • Featured Product
  • KD/KO Validated

DNAJB11 Polyclonal antibody

DNAJB11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15484-1-AP

Synonyms

ABBP 2, ABBP2, APOBEC1 binding protein 2, DJ9, DnaJ protein homolog 9, DNAJB11, EDJ, ER associated DNAJ, ER associated dnaJ protein 3, ERdj3, ERj3, ERj3p, hDj 9, hDj9, HEDJ, Human DnaJ protein 9, PRO1080, PWP1 interacting protein 4, UNQ537, UNQ537/PRO1080

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNAJB11 Polyclonal antibody (15484-1-AP)

    WB analysis using 15484-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15484-1-AP (DNAJB11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using DNAJB11 Polyclonal antibody (15484-1-AP)

    WB analysis of mouse heart using 15484-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 15484-1-AP (DNAJB11 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using DNAJB11 Polyclonal antibody (15484-1-AP)

    WB analysis of mouse liver using 15484-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15484-1-AP (DNAJB11 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using DNAJB11 Polyclonal antibody (15484-1-AP)

    IP experiment of mouse heart using 15484-1-AP

    IP result of anti-DNAJB11 (IP:15484-1-AP, 4ug; Detection:15484-1-AP 1:500) with mouse heart tissue lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using DNAJB11 Polyclonal antibody (15484-1-AP)

    IHC staining of human ovary tumor using 15484-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15484-1-AP (DNAJB11 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using DNAJB11 Polyclonal antibody (15484-1-AP)

    IHC staining of human ovary tumor using 15484-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15484-1-AP (DNAJB11 antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DNAJB11 Polyclonal antibody (15484-1-AP)

    IF Staining of HepG2 using 15484-1-AP

    Immunofluorescent analysis of HepG2 cells, using DNAJB11 antibody 15484-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, mouse heart tissue, HepG2 cells, rat liver tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15484-1-AP targets DNAJB11 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJB11 fusion protein Ag7832
Full Name DnaJ (Hsp40) homolog, subfamily B, member 11
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC001144
Gene Symbol DNAJB11
Gene ID (NCBI) 51726
RRIDAB_2094400
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DNAJB11 antibody 15484-1-APDownload protocol
IHC protocol for DNAJB11 antibody 15484-1-APDownload protocol
IF protocol for DNAJB11 antibody 15484-1-APDownload protocol
IP protocol for DNAJB11 antibody 15484-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Science

The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.

Authors - Michael J McKenna
View Article
humanWB

Cell

Defining Hsp70 Subnetworks in Dengue Virus Replication Reveals Key Vulnerability in Flavivirus Infection.

Authors - Shuhei Taguwa
  • KD Validated
View Article
mouseWB

Mol Cell

Differentiation Drives Widespread Rewiring of the Neural Stem Cell Chaperone Network.

Authors - Willianne I M Vonk
View Article
humanWB, IP

EMBO J

Unfolded protein response-induced ERdj3 secretion links ER stress to extracellular proteostasis.

Authors - Joseph C Genereux
  • KD Validated
View Article
humanWB

Cell Chem Biol

Quantitative Interactome Proteomics Reveals a Molecular Basis for ATF6-Dependent Regulation of a Destabilized Amyloidogenic Protein.

Authors - Lars Plate
View Article
humanWB

Cell Death Dis

Shutdown of ER-associated degradation pathway rescues functions of mutant iduronate 2-sulfatase linked to mucopolysaccharidosis type II.

Authors - Yosuke Osaki
  • KD Validated
View Article