DNAJA2 Polyclonal antibody
DNAJA2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 12236-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | A375 cells, mouse kidney tissue, human brain tissue |
| Positive IP detected in | A375 cells |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 8 publications below |
| IF | See 2 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
12236-1-AP targets DNAJA2 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DNAJA2 fusion protein Ag2877 |
| Full Name | DnaJ (Hsp40) homolog, subfamily A, member 2 |
| Calculated Molecular Weight | 417 aa, 46 kDa |
| Observed Molecular Weight | 46 kDa |
| GenBank Accession Number | BC015809 |
| Gene Symbol | DNAJA2 |
| Gene ID (NCBI) | 10294 |
| RRID | AB_2230709 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DNAJA2 antibody 12236-1-AP | Download protocol |
| IF protocol for DNAJA2 antibody 12236-1-AP | Download protocol |
| IP protocol for DNAJA2 antibody 12236-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Cancer Down-regulation of BiP/GRP78 sensitizes resistant prostate cancer cells to gene-therapeutic overexpression of REIC/Dkk-3. | ||
J Biol Chem A cytosolic chaperone complex controls folding and degradation of type III CD38. | ||
Cancers (Basel) Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin | ||
J Biol Chem Quantitative interactome proteomics identifies a proteostasis network for GABAA receptors | ||
Cancer Res N-myc-Mediated Translation Control Is a Therapeutic Vulnerability in Medulloblastoma |
