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DNAJA1 Polyclonal antibody
DNAJA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 11713-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, human testis tissue, HepG2 cells, human liver tissue, Jurkat cells, mouse lung tissue, mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 11 publications below |
| IF | See 4 publications below |
| IP | See 1 publications below |
Product Information
11713-1-AP targets DNAJA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DNAJA1 fusion protein Ag2284 |
| Full Name | DnaJ (Hsp40) homolog, subfamily A, member 1 |
| Calculated Molecular Weight | 397 aa, 45 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC008182 |
| Gene Symbol | DNAJA1 |
| Gene ID (NCBI) | 3301 |
| RRID | AB_10637266 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DNAJA1 is a member of the DnaJ proteins (also known as Hsp40 or Hsc40) which are cochaperones to DnaK (Hsp70) and function to modulate protein assembly, disassembly, and translocation. DNAJA1 has been reported to get involved in various processes, including virus infection, tau clearance, and mutant p53 degradation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DNAJA1 antibody 11713-1-AP | Download protocol |
| IHC protocol for DNAJA1 antibody 11713-1-AP | Download protocol |
| IF protocol for DNAJA1 antibody 11713-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Heat-shock chaperone HSPB1 regulates cytoplasmic TDP-43 phase separation and liquid-to-gel transition | ||
Nat Commun Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells. | ||
J Clin Invest Cul4A-DDB1-mediated monoubiquitination of phosphoglycerate dehydrogenase promotes colorectal cancer metastasis via increased S-adenosylmethionine.
| ||
Mol Ther Nucleic Acids Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunction | ||
Sci Rep Co-chaperones DNAJA1 and DNAJB6 are critical for regulation of polyglutamine aggregation.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Filip (Verified Customer) (10-28-2024) | I recommend this antibody. It does not recognize DNAJA2, which we run in parallel to DNAJA1.
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