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DNAI2 Polyclonal antibody

DNAI2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Cat no : 17533-1-AP

Synonyms

Dynein axonemal intermediate chain 2, CILD9

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse ovary tissue using DNAI2 Polyclonal antibody (17533-1-AP)

    WB analysis of mouse ovary using 17533-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 17533-1-AP (DNAI2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DNAI2 Polyclonal antibody (17533-1-AP)

    WB analysis of HeLa using 17533-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17533-1-AP (DNAI2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using DNAI2 Polyclonal antibody (17533-1-AP)

    WB analysis of mouse lung using 17533-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 17533-1-AP (DNAI2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using DNAI2 Polyclonal antibody (17533-1-AP)

    IP experiment of mouse lung using 17533-1-AP

    IP result of anti-DNAI2 (IP:17533-1-AP, 4ug; Detection:17533-1-AP 1:400) with mouse lung tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human heart tissue using DNAI2 Polyclonal antibody (17533-1-AP)

    IHC staining of human heart using 17533-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17533-1-AP (DNAI2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using DNAI2 Polyclonal antibody (17533-1-AP)

    IF Staining of mouse testis using 17533-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DNAI2 antibody (17533-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse ovary tissue, HeLa cells, mouse lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17533-1-AP targets DNAI2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAI2 fusion protein Ag11585
Full Name dynein, axonemal, intermediate chain 2
Calculated Molecular Weight 593 aa, 67 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC039582
Gene Symbol DNAI2
Gene ID (NCBI) 64446
RRIDAB_2096670
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DNAI2 antibody 17533-1-APDownload protocol
IHC protocol for DNAI2 antibody 17533-1-APDownload protocol
IF protocol for DNAI2 antibody 17533-1-APDownload protocol
IP protocol for DNAI2 antibody 17533-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF,IP

Elife

ZMYND10 functions in a chaperone relay during axonemal dynein assembly.

Authors - Girish R Mali
View Article
mouseIF

J Mol Cell Biol

Production of transgenic mice by random recombination of targeted genes in female germline stem cells.

Authors - Zhang Yong Y
View Article
ratWB,IF

Front Cell Dev Biol

PIH1D3-knockout rats exhibit full ciliopathy features and dysfunctional pre-assembly and loading of dynein arms in motile cilia

Authors - Tingting Zhang
View Article
mouseWB

Development

CCDC183 is essential for cytoplasmic invagination around the flagellum during spermiogenesis and male fertility

Authors - Keisuke Shimada
View Article
mouseWB

Exp Anim

The motor domain of testis-enriched kinesin KIF9 is essential for its localization in the mouse flagellum.

Authors - Haruhiko Miyata
View Article
humanWB

Ann Hum Genet

DNAH17 is associated with asthenozoospermia and multiple morphological abnormalities of sperm flagella.

Authors - Yanwei Sha
View Article