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DNA2 Polyclonal antibody

DNA2 Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21599-1-AP

Synonyms

DNA2, DNA2L, KIAA0083

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HEK-293 cells using DNA2 Polyclonal antibody (21599-1-AP)

    IP experiment of HEK-293 using 21599-1-AP

    IP result of anti-DNA2 (IP:21599-1-AP, 4ug; Detection:21599-1-AP 1:300) with HEK-293 cells lysate 2600ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DNA2 Polyclonal antibody (21599-1-AP)

    IHC staining of human liver cancer using 21599-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21599-1-AP (DNA2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DNA2 Polyclonal antibody (21599-1-AP)

    IHC staining of human liver cancer using 21599-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21599-1-AP (DNA2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using DNA2 Polyclonal antibody (21599-1-AP)

    IHC staining of human cervical cancer using 21599-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21599-1-AP (DNA2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using DNA2 Polyclonal antibody (21599-1-AP)

    IHC staining of human cervical cancer using 21599-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21599-1-AP (DNA2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21599-1-AP is DNA2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNA2 fusion protein Ag16206
Full Name DNA replication helicase 2 homolog (yeast)
Calculated Molecular Weight 1060 aa, 120 kDa
Observed Molecular Weight 125 kDa
GenBank Accession NumberBC111740
Gene Symbol DNA2
Gene ID (NCBI) 1763
RRIDAB_2878890
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA2(DNA replication ATP-dependent helicase/nuclease DNA2) is also named as DNA2L, KIAA0083 and belongs to the DNA2/NAM7 helicase family. This protein includes 2 domains: DNA replication nuclease and DNA replication ATP-dependent helicase. It is a conserved helicase/nuclease involved in the maintenance of mitochondrial and nuclear DNA stability and it can interact with DNA polymerase gamma and stimulating its activity. It can also process intermediate 5' flap structures occuring in DNA replication long-patch base excision repair in mitochondria. It has 4 isoforms produced by alternative splicing. This antibody is specific to DNA2.

Protocols

Product Specific Protocols
IHC protocol for DNA2 antibody 21599-1-APDownload protocol
IP protocol for DNA2 antibody 21599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols