Tested Applications
| Positive WB detected in | mouse liver tissue, mouse thymus tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 8 publications below |
| WB | See 10 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
18727-1-AP targets DNA2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | DNA replication helicase 2 homolog (yeast) |
| Calculated Molecular Weight | 120 kDa |
| Observed Molecular Weight | 120-130 kDa |
| GenBank Accession Number | NM_001080449 |
| Gene Symbol | DNA2 |
| Gene ID (NCBI) | 1763 |
| RRID | AB_10637998 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51530 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DNA2(DNA replication ATP-dependent helicase/nuclease DNA2) is also named as DNA2L, KIAA0083 and belongs to the DNA2/NAM7 helicase family. This protein includes 2 domains: DNA replication nuclease and DNA replication ATP-dependent helicase. It is a conserved helicase/nuclease involved in the maintenance of mitochondrial and nuclear DNA stability and it can interact with DNA polymerase gamma and stimulating its activity. It can also process intermediate 5' flap structures occuring in DNA replication long-patch base excision repair in mitochondria. It has 4 isoforms produced by alternative splicing. This antibody is specific to DNA2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DNA2 antibody 18727-1-AP | Download protocol |
| IHC protocol for DNA2 antibody 18727-1-AP | Download protocol |
| IF protocol for DNA2 antibody 18727-1-AP | Download protocol |
| IP protocol for DNA2 antibody 18727-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Stalled replication fork protection limits cGAS-STING and P-body-dependent innate immune signalling.
| ||
Mol Cell Dynamic control of RNA-DNA hybrid formation orchestrates DNA2 activation at stalled forks by RNAPII and DDX39A
| ||
Genes Dev Abro1 maintains genome stability and limits replication stress by protecting replication fork stability.
| ||
Commun Biol The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells
| ||
Cell Cycle The checkpoint clamp protein Rad9 facilitates DNA-end resection and prevents alternative non-homologous end joining.
| ||
Cells Increased Resection at DSBs in G2-Phase Is a Unique Phenotype Associated with DNA-PKcs Defects That Is Not Shared by Other Factors of c-NHEJ.
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