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DMRTC1B Polyclonal antibody

DMRTC1B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21473-1-AP

Synonyms

DMRT like family C1B, DMRTC1, DMRTC1B

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using DMRTC1B Polyclonal antibody (21473-1-AP)

    WB analysis of MCF-7 using 21473-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 21473-1-AP (DMRTC1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using DMRTC1B Polyclonal antibody (21473-1-AP)

    WB analysis of mouse spleen using 21473-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21473-1-AP (DMRTC1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using DMRTC1B Polyclonal antibody (21473-1-AP)

    WB analysis of MCF-7 using 21473-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 21473-1-AP (DMRTC1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inMCF7 cells, MCF-7 cells, mouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21473-1-AP is DMRTC1B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DMRTC1B fusion protein Ag15419
Full Name DMRT-like family C1B
Calculated Molecular Weight 192 aa, 20 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC029799
Gene Symbol DMRTC1B
Gene ID (NCBI) 728656
RRIDAB_11064607
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DMRTC1B, also named as Doublesex- and mab-3-related transcription factor C1, is a 192 amino acid protein, which belongs to the DMRT family. DMRTC1B is predominantly expressed in kidney, pancreas, ovary and testis. Detected in brain and in many other tissues. DMRTC1B exists two isoform and molecular weight of isoforms are 20 kDa and 13 kDa.

Protocols

Product Specific Protocols
WB protocol for DMRTC1B antibody 21473-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols