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DMRTA1 Polyclonal antibody

DMRTA1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 24356-1-AP

Synonyms

DMO, DMRT like family A1, DMRTA1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using DMRTA1 Polyclonal antibody (24356-1-AP)

    WB analysis of mouse liver using 24356-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24356-1-AP (DMRTA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using DMRTA1 Polyclonal antibody (24356-1-AP)

    IP experiment of mouse liver using 24356-1-AP

    IP result of anti-DMRTA1 (IP:24356-1-AP, 3ug; Detection:24356-1-AP 1:500) with mouse liver tissue lysate 3000ug.

Tested Applications

Positive WB detected inmouse liver tissue
Positive IP detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24356-1-AP is DMRTA1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DMRTA1 fusion protein Ag18796
Full Name DMRT-like family A1
Calculated Molecular Weight 504 aa, 53 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC130437
Gene Symbol DMRTA1
Gene ID (NCBI) 63951
RRIDAB_2879506
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DMRTA1 antibody 24356-1-APDownload protocol
IP protocol for DMRTA1 antibody 24356-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols