• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

DMKN Polyclonal antibody

DMKN Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 16252-1-AP

Synonyms

dermokine, DMKN, UNQ729, ZD52F10

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse ovary tissue using DMKN Polyclonal antibody (16252-1-AP)

    WB analysis of mouse ovary using 16252-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16252-1-AP (DMKN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using DMKN Polyclonal antibody (16252-1-AP)

    WB analysis of mouse lung using 16252-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16252-1-AP (DMKN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using DMKN Polyclonal antibody (16252-1-AP)

    IP experiment of mouse lung using 16252-1-AP

    IP result of anti-DMKN (IP:16252-1-AP, 4ug; Detection:16252-1-AP 1:500) with mouse lung tissue lysate 4000ug.

Tested Applications

Positive WB detected inmouse ovary tissue, mouse lung tissue
Positive IP detected inmouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16252-1-AP targets DMKN in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DMKN fusion protein Ag9242
Full Name dermokine
Calculated Molecular Weight 476 aa, 47 kDa
Observed Molecular Weight 48-55 kDa
GenBank Accession NumberBC011886
Gene Symbol DMKN
Gene ID (NCBI) 93099
RRIDAB_10596473
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DMKN, also named as epidermis-specific secreted protein SK30/SK89, acts as a soluble regulator of keratinocyte differentiation. DMKN has 16 isoforms with MW 9-20 kDa, 35-38 kDa and 42-48 kDa.

Protocols

Product Specific Protocols
WB protocol for DMKN antibody 16252-1-APDownload protocol
IP protocol for DMKN antibody 16252-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell Proteomics

Deletion of cysteine cathepsins B or L yields differential impacts on murine skin proteome and degradome.

Authors - Tholen Stefan S
View Article

Mol Cell Proteomics

Matrix metalloproteinase 10 degradomics in keratinocytes and epidermal tissue identifies bioactive substrates with pleiotropic functions.

Authors - Pascal Schlage
View Article