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  • Featured Product
  • KD/KO Validated

DLGAP3 Polyclonal antibody

DLGAP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55056-1-AP

Synonyms

PSD-95/SAP90-binding protein 3, PSD 95/SAP90 binding protein 3, Disks large-associated protein 3, DAP-3, DAP3

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using DLGAP3 Polyclonal antibody (55056-1-AP)

    WB analysis of human brain using 55056-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 55056-1-AP (DLGAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using DLGAP3 Polyclonal antibody (55056-1-AP)

    WB analysis of mouse brain using 55056-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55056-1-AP (DLGAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using DLGAP3 Polyclonal antibody (55056-1-AP)

    IHC staining of human stomach using 55056-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55056-1-AP (DLGAP3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman brain tissue, mouse brain tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55056-1-AP targets DLGAP3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name discs, large (Drosophila) homolog-associated protein 3
Calculated Molecular Weight 106 kDa
Observed Molecular Weight 106-120 kDa
GenBank Accession NumberNM_001080418
Gene Symbol DLGAP3
Gene ID (NCBI) 58512
RRIDAB_10858793
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DLGAP3, also named as DAP3, belongs to the SAPAP family. It may play a role in the molecular organization of synapses and neuronal cell signaling. DLGAP3 could be an adapter protein linking ion channel to the subsynaptic cytoskeleton. It may induce enrichment of PSD-95/SAP90 at the plasma membrane. The antibody has no cross reaction to other DLGAP members.

Protocols

Product Specific Protocols
WB protocol for DLGAP3 antibody 55056-1-APDownload protocol
IHC protocol for DLGAP3 antibody 55056-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Biol Psychiatry

Metabotropic Glutamate Receptor and Fragile X Signaling in a Female Model of Escalated Aggression.

Authors - Laura E Been
View Article
human,mouseWB,IF

Cell Death Dis

SAPAP3 regulates epileptic seizures involving GluN2A in post-synaptic densities.

Authors - Yanke Zhang
  • KD Validated
View Article
mouseWB

Nat Neurosci

Neuronal activity rapidly reprograms dendritic translation via eIF4G2:uORF binding

Authors - Ezgi Hacisuleyman
View Article