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DLEC1 Polyclonal antibody

DLEC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20027-1-AP

Synonyms

DLC1, F56, DLC-1, DLC 1

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using DLEC1 Polyclonal antibody (20027-1-AP)

    WB analysis of human testis using 20027-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 20027-1-AP (DLEC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using DLEC1 Polyclonal antibody (20027-1-AP)

    WB analysis of PC-3 using 20027-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20027-1-AP (DLEC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using DLEC1 Polyclonal antibody (20027-1-AP)

    WB analysis of A549 using 20027-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 20027-1-AP (DLEC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of PC-3 cells using DLEC1 Polyclonal antibody (20027-1-AP)

    IP experiment of PC-3 using 20027-1-AP

    IP result of anti-DLEC1 (IP:20027-1-AP, 4ug; Detection:20027-1-AP 1:300) with PC-3 cells lysate 1100ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using DLEC1 Polyclonal antibody (20027-1-AP)

    IHC staining of human testis using 20027-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20027-1-AP (DLEC1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using DLEC1 Polyclonal antibody (20027-1-AP)

    IHC staining of human testis using 20027-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20027-1-AP (DLEC1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using DLEC1 Polyclonal antibody (20027-1-AP)

    IF Staining of PC-3 using 20027-1-AP

    Immunofluorescent analysis of PC-3 cells using 20027-1-AP (DLEC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman testis tissue, A549 cells, PC-3 cells
Positive IP detected inPC-3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20027-1-AP targets DLEC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name deleted in lung and esophageal cancer 1
Calculated Molecular Weight 196 kDa
Observed Molecular Weight 80-90 kDa, 46 kDa
GenBank Accession NumberNM_007335
Gene Symbol DLEC1
Gene ID (NCBI) 9940
RRIDAB_10664924
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DLEC1, also named as DLC1 F56, may act as a tumor suppressor by inhibiting cell proliferation.

Protocols

Product Specific Protocols
WB protocol for DLEC1 antibody 20027-1-APDownload protocol
IHC protocol for DLEC1 antibody 20027-1-APDownload protocol
IF protocol for DLEC1 antibody 20027-1-APDownload protocol
IP protocol for DLEC1 antibody 20027-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols